Angebote zu "Filtration" (70 Treffer)

Kategorien

Shops

Antitumor Activity of L-Asparaginase from Chick...
49,00 € *
ggf. zzgl. Versand

This study aimed to isolate, purify and characterize L-asparaginase present in some animal tissues. The L-asparaginase (EC 3.5.1.1) produced by chicken livers was isolated and characterized. Different purification steps (including ammonium sulphate fractionation followed by separation on Sephadex G-100 gel filtration and Sephadex G-200 gel filtration) were applied to crude filtrate to obtain a pure enzyme preparation. The enzyme was purified 128.5 ± 0.5 fold and showed a final specific activity of 158.11 ± 5.0 U/mg with a 17.1 ± 8.6 % yield. Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) of the purified enzyme revealed it was one peptide chain with Mr of 33 kDa while by gel filtration appears to be 36 kDa. The enzyme was very specific to L-asparagine and did not hydrolyze L-glutamine. A Lineweaver-Burk analysis showed a Km value of 1.66 mM toward L-asparagine as substrate and Vmax of 34.47 U. The enzyme showed maximum activity at pH 11.0 when incubated at 60 C for 20 min. The amino acids composition of the purified enzyme was also determined. Antitumor activity was investigated. The enzyme inhibited the growth of the two human cell lines including hepatoc

Anbieter: Dodax
Stand: 24.01.2020
Zum Angebot
Antitumor Activity of L-Asparaginase from Chick...
50,40 € *
ggf. zzgl. Versand

This study aimed to isolate, purify and characterize L-asparaginase present in some animal tissues. The L-asparaginase (EC 3.5.1.1) produced by chicken livers was isolated and characterized. Different purification steps (including ammonium sulphate fractionation followed by separation on Sephadex G-100 gel filtration and Sephadex G-200 gel filtration) were applied to crude filtrate to obtain a pure enzyme preparation. The enzyme was purified 128.5 ± 0.5 fold and showed a final specific activity of 158.11 ± 5.0 U/mg with a 17.1 ± 8.6 % yield. Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) of the purified enzyme revealed it was one peptide chain with Mr of 33 kDa while by gel filtration appears to be 36 kDa. The enzyme was very specific to L-asparagine and did not hydrolyze L-glutamine. A Lineweaver-Burk analysis showed a Km value of 1.66 mM toward L-asparagine as substrate and Vmax of 34.47 U. The enzyme showed maximum activity at pH 11.0 when incubated at 60 C for 20 min. The amino acids composition of the purified enzyme was also determined. Antitumor activity was investigated. The enzyme inhibited the growth of the two human cell lines including hepatoc

Anbieter: Dodax AT
Stand: 24.01.2020
Zum Angebot
Determann, Helmut: Gel Chromatography
87,69 € *
ggf. zzgl. Versand

Erscheinungsdatum: 01.01.1969, Medium: Taschenbuch, Einband: Kartoniert / Broschiert, Titel: Gel Chromatography, Titelzusatz: Gel Filtration · Gel Permeation · Molecular Sieves A Laboratory Handbook, Auflage: 2. Auflage von 1969 // 2nd ed. 1969. Softcover reprint of the original 2nd ed. 1969, Autor: Determann, Helmut, Verlag: Springer Berlin Heidelberg // Springer Berlin, Sprache: Englisch, Schlagworte: Biochemie, Rubrik: Theoretische Chemie, Seiten: 220, Informationen: Paperback, Gewicht: 341 gr, Verkäufer: averdo

Anbieter: averdo
Stand: 24.01.2020
Zum Angebot
Size Exclusion Chromatography
35,00 € *
ggf. zzgl. Versand

High Quality Content by WIKIPEDIA articles! Size exclusion chromatography (SEC) is a chromatographic method in which molecules in solution are separated based on their size (more correctly, their hydrodynamic volume). It is usually applied to large molecules or macromolecular complexes such as proteins and industrial polymers. Typically, when an aqueous solution is used to transport the sample through the column, the technique is known as gel filtration chromatography, versus the name gel permeation chromatography which is used when an organic solvent is used as a mobile phase. SEC is a widely used Polymer characterization method because of its ability to provide good Mw results for polymers.

Anbieter: Dodax AT
Stand: 24.01.2020
Zum Angebot
Size Exclusion Chromatography
34,00 € *
ggf. zzgl. Versand

High Quality Content by WIKIPEDIA articles! Size exclusion chromatography (SEC) is a chromatographic method in which molecules in solution are separated based on their size (more correctly, their hydrodynamic volume). It is usually applied to large molecules or macromolecular complexes such as proteins and industrial polymers. Typically, when an aqueous solution is used to transport the sample through the column, the technique is known as gel filtration chromatography, versus the name gel permeation chromatography which is used when an organic solvent is used as a mobile phase. SEC is a widely used Polymer characterization method because of its ability to provide good Mw results for polymers.

Anbieter: Dodax
Stand: 24.01.2020
Zum Angebot
Xylanases of Fungi from Extreme Habitats in Egypt
82,90 € *
ggf. zzgl. Versand

In this book, the following methods and techniques are described and illustrated in details: 1- The methods for chemical analyses of different water, mud and soil samples. 2- Isolation of mesophilic, halotolerant/halophilic and thermotolerant/thermophilic fungi from air, water, mud and soil. 3- Extraction of xylan from different sources and its assay by UV-spectroscopy and HPLC. 4- Screening the potential of different fungi to produce xylanases. 5- Optimization of nutritional and environmental factors for the maximization of xylanases production. 6- Different techniques of xylanases production such as solid-state and submerged fermentation. 7- Determination of relative and specific activities as well as the total protein for carboxy methyl cellulase, avicellase and xylanase enzymes. 8- Purification processes of xylanase enzyme such as: ammonium sulfate precipitation, dialysis, ion-exchange column chromatography and gel filtration column chromatography. 9- Characterization of xylanase enzyme.

Anbieter: Dodax
Stand: 24.01.2020
Zum Angebot
Synthesis of Biginelli reaction products using ...
39,90 € *
ggf. zzgl. Versand

In my work I have focused on the various aspects of Biginelli reaction using a very promising and effective heterogeneous catalyst. The catalyst having acidic property and eco-friendly nature which is derived from thiomaleic anhydride composited with silica gel porous material for three component one-pot Biginelli reactions. Total seven biginellibased reactions were performed and all the products were obtained with good yields. This bronsted acid catalyst works efficiently at room temperature or with some heating without refluxing and high yielding in lesser time. This single step and one-pot synthesis takes almost two hours to complete the reactions. Product separation is simple just by filtration. Very low amount of organic solvent is required to conduct the reactions. Catalyst is characterized by BET analysis. NMR and IRcharacterizations are done with all the seven obtained products. All the reactions progress will be controlled by thin layer chromatography methods and the measurements of melting points of all the dried products.

Anbieter: Dodax
Stand: 24.01.2020
Zum Angebot
Methodology for Extraction and Purification of ...
50,40 € *
ggf. zzgl. Versand

Mutarotases are recently characterized group of enzymes that are involved in anomeric conversions of monosaccharides enhancing rate of metabolism. Mutarotase was extracted and purified from bovine kidney cortex by (NH4)2SO4 precipitation technique, dialysis and gel filtration chromatography. Polarimeter was used for assay of Mutarotase in this present project. Mutarotase increased activity from 0.478 to 1.711 U/ml by purification. It gained 13.4 folds purification at this final step. It is helpful in determining the glucose level and participates in glycolysis and gluconeogenesis. Activities and fold purification of Mutarotase can also be increased by much improved techniques of purification like ion exchange chromatography or FPLC hence enhancing the production of this enzyme.

Anbieter: Dodax AT
Stand: 24.01.2020
Zum Angebot
Lipase production from haloalkaliphilic bacteria
41,10 € *
ggf. zzgl. Versand

A study was conducted for isolation and characterization for lipases(cheap and versatile catalysts to degrade lipids in more modern applications) from hyperalkaline and hypersaline soil. Amongst 15 isolated strains, 9 isolates were screened for lipase production and further study was followed by screening of best lipase producers. In addition, lipase production was also measured in presence of various parameters viz. different lipidic carbon sources, metal ions, temperature, pH to optimize the medium. Efforts have been made to purify the protein by ammonium sulphate precipitation, gel filtration, and ion exchange chromatography. However, in the future we can consider genetical characterization and selection of the most desirable strains, which can assess their potential as starter cultures for commercial use.

Anbieter: Dodax AT
Stand: 24.01.2020
Zum Angebot